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Coal to gas single cell protein-contribute to resolving the food crisis of China in future

Cao Chen,Yu Bo and Gu Weidong

Strategic Study of CAE 2015, Volume 17, Issue 3,   Pages 107-112

Abstract: model that by combining non-grid-connected wind power and coal to gas with bioengineering to produce singlecell protein to contribute to resolving the food crisis.By combining non-grid-connected wind power and coal to gas with bioengineering to produce single cellprotein can not only use the coal resource more efficiently， environmental friendly， but also producenutrient rich protein for forage and human food.

Keywords： urbanization； food crisis； non-grid wind power； coal-to-gas； single cell protein    

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structureinvolves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts.In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein.

Keywords： cell surface engineering     surface display     whole-cell catalysts     bioprocess    

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Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 224-229 doi: 10.1007/s11705-008-0029-9

Abstract: In the new era of “Omics”, the traditional techniques of protein expression in vivo can not come up withThe cell-free protein synthesis system provides a new strategy of protein expression with advantagessystem are the key factors for the successful construction of cell-free protein expression system.The cell growth phase, the pressure for cell disruption and the storage condition of cell extracts wereUnder the optimized conditions, the green fluorescent protein (GFP) reporter gene was expressed in the

Keywords： cell-free     regeneration     extract     information     successful construction    

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Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell

Frontiers of Medicine doi: 10.1007/s11684-023-1017-7

Abstract: By conducting single-cell RNA-seq analyses on 26 gliomas, we reported their classification into primitiveoligodendrocyte precursor cell (pri-OPC)-like and radial glia (RG)-like tumors and validated it in aFor example, glial/glioma stem cell markers OLIG1/PTPRZ1 and B cell-specific receptors

Keywords： single-cell RNA-seq     glioma     radial glia     primitive oligodendrocyte precursor cell     immune escape    

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Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation of membrane proteins

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1188-2

Abstract: Single cell analysis technology wereused to study the morphological structure.the adsorption, uptake and transmembrane transport of PAHs by bacteria, the regulation of membrane proteinMeanwhile, introduce the proteomics and single cell analysis technology used to address these areas ofAdditionally, analyze the changes in morphology and structure and the characteristics of microbial cellFinally, recognize the microscopic mechanism of PAHs biodegradation in terms of cell and membrane proteins

Keywords： Transmembrane transport     Adsorption and uptake of hydrocarbons     Proteomics     Functional regulation of membrane protein     Single cell analysis technology    

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Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

Frontiers of Medicine   Pages 939-956 doi: 10.1007/s11684-023-0987-9

Abstract: end, 12 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cellMNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell–cell Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4single-cell transcriptomic data.In conclusion, a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled, highlighting

Keywords： mononuclear diploid cardiomyocytes     cell–cell communication     cardiac fibroblast     single-cell RNA sequencing    

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PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 251-262 doi: 10.1007/s11684-021-0915-9

Abstract: Taking advantage of single-cell RNA-sequencing (scRNA-seq) analysis, we can assess the transcriptomicfeatures at the single-cell level.Still, the tools used to interpret pathogens (such as viruses, bacteria, and fungi) at the single-cellcomputational pipeline that uses unmapped scRNA-seq data to identify intracellular pathogens at the single-cellthe state-of-the-art tools such as Viral-Track, and the first tools for identifying bacteria at the single-cell

Keywords： scRNA-seq     intracellular pathogen     microbe     COVID-19     SARS-CoV-2    

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Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 1,   Pages 90-97 doi: 10.1007/s11705-018-1766-z

Abstract: into physiological environments and driven by energetics, they readily associate proteins forming a proteinsurface characteristics, affecting their interaction with cells due to conformational changes in adsorbed proteinapproach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cellthe surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein

Keywords： ZnONPs     nanoparticles     protein corona     ClueGO    

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Analysis of statistical thermodynamic model for binary protein adsorption equilibria on cation exchange

ZHOU Xiaopeng, SU Xueli, SUN Yan

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 2,   Pages 103-112 doi: 10.1007/s11705-007-0020-x

Abstract: This work describes the nonlinear binary protein adsorption equilibria on ion exchangers by the statisticalThe single-component and binary protein adsorption isotherms of bovine hemoglobin (Hb) and bovine serumThe ST model was found to depict the effects of pH and ionic strength on the single-component equilibriaMoreover, the ST model gave acceptable fitting to the binary adsorption data with the fitted single-componentResults demonstrate the availability of the ST model for describing nonlinear competitive protein adsorption

Keywords： fundamental importance     single-component equilibria     acceptable fitting     hemoglobin     chromatographic separation    

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Clinical characteristics and prognostic factors of patients with mature T-cell lymphoid malignancies:a single-institution study of 225 cases

Wen Xue,Yan Sheng,Xiangqin Weng,Yongmei Zhu,Yan Zhao,Pengpeng Xu,Xiaochun Fei,Xiaoyan Chen,Li Wang,Weili Zhao

Frontiers of Medicine 2015, Volume 9, Issue 4,   Pages 468-477 doi: 10.1007/s11684-015-0419-6

Abstract:

Mature T-cell lymphoid malignancies comprise a group of heterogeneous diseases that vary in clinicopathologicalBone marrow (BM) infiltration is more commonly present in mature T-cell lymphoid malignancies comparedwith their B-cell counterparts and hence important for differential diagnosis.lymphoid malignancies treated in a single institution.These included 29 cases of T-cell lymphoproliferative disorders (T-LPD, all with BM infiltration) and

Keywords： mature T-cell lymphoid malignancies     clonal T-cell population     bone marrow infiltration     prognostic factors    

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Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

Frontiers of Medicine 2010, Volume 4, Issue 1,   Pages 106-111 doi: 10.1007/s11684-010-0004-y

Abstract: Hepatitis B virus X protein (HBx), a 17-kd protein encoded by X gene of hepatitis B virus (HBV), hasThe aim of the study is to investigate the extracellular regulated protein kinases (ERKs) pathway ofHBx on glomerular mesangial cell (GMC) proliferation and tumor necrosis factor-α (TNF-α) expressioncan conclude that HBx could induce GMC proliferation and increase TNF-α mRNA expression and its proteinHBx upregulates TNF-α expression and induces cell proliferation of GMC line partly through ERK signal

Keywords： hepatitis B virus     X gene     glomerular mesangial cell line     extracellular regulated protein kinases     tumor    

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Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 26-29 doi: 10.1007/s11684-009-0008-7

Abstract: human cervical carcinoma cell lines HeLa, SiHa, C33A, Caski, three human breast carcinoma cell linesassay, and western blot was applied to detect the expressions of Ku80, DNA-PKcs and ATM protein.The correlativity of protein expression with SF2 value was analyzed by Pearson linear correlation analysisWe found that the expression of same protein in different cell lines and the expression of three proteinsThese findings suggest that the expression level of DNA-PKcs protein can be an indicator for predicting

Keywords： Ku80     DNA-PK(cs)-binding protein     human     ataxia telangiectasia mutated protein     tumor cell lines     radiosensitivity    

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Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Frontiers of Medicine 2021, Volume 15, Issue 1,   Pages 144-154 doi: 10.1007/s11684-020-0792-7

Abstract: vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell

Keywords： human metaphase II oocyte     vitrification     cryostorage duration     single-cell RNA-Seq     lncRNA    

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Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor-induced cell migration

GONG Xiaowei, WEI Jie, LI Yusheng, CHENG Weiwei, DENG Peng, JIANG Yong

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 248-252 doi: 10.1007/s11684-007-0047-x

Abstract: The aim of this study was to investigate the role of p38 mitogen-activated protein kinase (MAPK) in cellA Transwell cell migration system was used to determine the effects of PDGF treatment on the migrationand the influence of deficiency on this process in a gene knockout (p38) mouse embryonic fibroblast cellFurthermore, the PDGF-induced cell migration was significantly blocked in gene knockout (p38) mouse) as compared with the wild type cells (p38). p38 MAPK plays an important role in the regulation of cell

Keywords： control     stimulation     mitogen-activated     growth factor     process    

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A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu, Shihui Wang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 2,   Pages 410-420 doi: 10.1007/s11705-020-1950-9

Abstract: Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.

Keywords： GO     yeast     polyelectrolyte     cytoprotection     nanomaterials    

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Title Author Date Type Operation

Coal to gas single cell protein-contribute to resolving the food crisis of China in future

Cao Chen,Yu Bo and Gu Weidong

Journal Article

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Journal Article

Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Journal Article

Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell

Journal Article

Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation of membrane proteins

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

Journal Article

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

Journal Article

PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

Journal Article

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

Journal Article

Analysis of statistical thermodynamic model for binary protein adsorption equilibria on cation exchange

ZHOU Xiaopeng, SU Xueli, SUN Yan

Journal Article

Clinical characteristics and prognostic factors of patients with mature T-cell lymphoid malignancies:a single-institution study of 225 cases

Wen Xue,Yan Sheng,Xiangqin Weng,Yongmei Zhu,Yan Zhao,Pengpeng Xu,Xiaochun Fei,Xiaoyan Chen,Li Wang,Weili Zhao

Journal Article

Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

Journal Article

Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

Journal Article

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Journal Article

Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor-induced cell migration

GONG Xiaowei, WEI Jie, LI Yusheng, CHENG Weiwei, DENG Peng, JIANG Yong

Journal Article

A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu, Shihui Wang

Journal Article